The usefulness of the reported quantity in a food matrix relies on the fact that it should maintain the same chemical condition as that responsible for the current response. Therefore, this work seeks to explore whether the NiFe nanowire chronoamperometry can be able to report three kinds of output from a food sample, which include a single concentration for a specific reducing-sugar class, a range of concentration for an unclassifiable reducing-sugar class, and the total sugar content after conversion. In doing so, the calculations will employ calibration and validation values for glucose, fructose, galactose, lactose, maltose, sucrose after pre-treatment, and food samples of peach juice, honey, milk, isotonic solution, coke, diet coke, and apple. These values will be organized using RLC, CBCE, HGP, and RTA without adding a new laboratory procedure. The first common linear calibration interval of these sugars and foods was between 0.05 mM and 0.30 mM. Glucose, fructose, and galactose belong to the high responding monosaccharides having the mean sensitivity of 0.6427 μA μM−1 cm−2, and lactose, and maltose belong to the low responding disaccharides with a mean sensitivity of 0.3555 μA μM−1 cm−2. Consequently, the class multiplier becomes 1.81, implying that an unknown class current difference can only be reported as a concentration after specifying its chemical condition. Monosaccharides and disaccharides have mean RLC of 0.9168 and 0.6175 respectively. The mean recovery values in the food validation were 98.05% for reducing sugar and 99.11% for total sugar. Hydrolysis contribution of the peach-juice and apple was 67.36% and 38.44% respectively. Therefore, the response by the NiFe nanowire is fit for food quality determination when the reported value is clearly specified with regard to sugar class and conversion state.